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dna sequencing analysis software program version 5.1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dna sequencing analysis software program version 5.1
    Dna Sequencing Analysis Software Program Version 5.1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/software+program+for+dna+sequencing/sequencing+analysis+software+v5+3+1/pmc07865561-232-5-12
    Average 90 stars, based on 1 article reviews
    dna sequencing analysis software program version 5.1 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Recombinant:

    Article Title: The Developmental and Genetic Architecture of the Sexually Selected Male Ornament of Swordtails.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins TRIzol Reagent Invitrogen Cat#15596026 dNTPs (Set, 100mM) Sigma-Aldrich Cat#DNTP100 DNase, RNase-free Thermo Scientific Cat#EN0521 SYBR Green I (10 000x in DMSO) Invitrogen Cat#S7563 Q5 High Fidelity DNA Polymerase New England Biolabs Cat#M04915 Critical Commercial Assays AmpliCap-Max T7 High Yield Message Maker Kit Biozym Cat#150472 RevertAid First Strand cDNA Synthesis Kit Thermo Scientific Cat#K1621 Deposited Data kcnh8 sequences this paper https://github.com/dukecomeback/swordtail/blob/main/ kcnh8_Xiphophorus.fa RNA-seq short read sequences 16 and this paper ENA accession code PRJEB8012 and PRJNA673149 Experimental Models: Organisms/Strains Xiphophorus hellerii, Rio Lancetilla strain Biocenter Würzburg WLC1337 Xiphophorus maculatus, Rio Jamapa strain Jp163A Biocenter Würzburg WLC6628 Xiphophorus montezumae, Tamasops strain Biocenter Würzburg WLC1052 Xiphophorus monticolus, El Tejon strain Biocenter Würzburg WLC3344 Xiphophorus pygmaeus, strain Rio Axtla Biocenter Würzburg WLC3015 Priapella lacandonae Biocenter Würzburg WLC5281 Oryzias latipes, Carbio strain Biocenter Würzburg WLC2674 Oligonucleotides Oligonucleotide Primers for PCR see Table S3 for primers N/A Recombinant DNA pcDNA3 Thermo Fisher V79020 pNBI16/pNB1u 66 N/A Software and Algorithms GSNAP version 2018-03-25 67 http://research-pub.gene.com/gmap/ STAR 66 https://github.com/alexdobin/STAR/releases DESeq2 68 https://bioconductor.org/packages/release/bioc/html/ DESeq2.html Ensembl Biomart 69 http://www.ensembl.org/biomart/ martview/ a61c56ebb44f17 c0e39ce71a4d79f44a DAVID 70 https://david.ncifcrf.gov/ Inparanoid 71 http://inparanoid.sbc.su.se/cgi-bin/index.cgi Pal2Nal 72 http://www.bork.embl.de/pal2nal/ MUSCLE 73 https://www.ebi.ac.uk/Tools/msa/muscle/ Gblocks 74 http://phylogeny.lirmm.fr/phylo_cgi/one_task.cgi?task_ type=gblocks Phylip 75 http://evolution.genetics.washington.edu/phylip.html, FigTree see website http://tree.bio.ed.ac.uk/software/figtree/ ETE3 76 http://etetoolkit.org/ Primer3 (version 4.4.0) see website https://bioinfo.ut.ee/primer3/ R/qtl v.1.39-5 see website https://rqtl.org/ (Continued on next page) Current Biology 31, 911–922.e1–e4, March 8, 2021 e1 ..

    Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
    Article Snippet: Reagent or resource Source Identifier Antibodies Rabbit Monoclonal Anti-SOX9 Abcam Cat# ab185230, RRID: AB_2715497 Rabbit Monoclonal Anti-CDH1 Cell Signaling Cat#: 3195, RRID: AB_2291471 Biotinylated Goat Anti-rabbit IgG Vector Labs Cat#: BA-1000, RRID: AB_2313606 Horse Anti-rabbit IgG, ImmPRESS VR Reagent Vector Labs Cat#: MP-6401, RRID: AB_2336529 Bacterial and Virus Strains None Biological Samples Goat Serum Millipore-Sigma Cat#: G9023 Bovine Serum Albumin Millipore-Sigma Cat#: 29-102-5 GM Trypsin Difco Cat#: 215240 Collagenase Millipore-Sigma Cat#: C9891 Chemicals, Peptides, and Recombinant Proteins Bluo-gal Gold Biotechnology Cat#: B-673-250 Critical Commercial Assays Agilent RNA 6000 Pico Agilent Technologies Cat#: 5067-1513 GeneChip Mouse Gene 1.0 ST Array ThermoFisher Scientific Cat#: 901171 Deposited Data RNA Microarray Data NCBI GEO Database GSE113011 ; reviewer token mhizgciwxxcxbyl Experimental Models: Cell Lines None Experimental Models: Organisms/Strains Mouse: C57BL/6J Jackson Laboratory Stock#:000664, RRID: IMSR_JAX: 000664 Mouse: B6.129S4-Gt(ROSA)26 sortm1Sor /J Jackson Laboratory Stock# 003474, RRID: IMSR_J AX: 003474 Mouse: B6. .. CgShh tm1(EGFP/cre)Cjt /J Jackson Laboratory Stock#005622; RRID: IMSR_J AX: 005622 Mouse: B6.129S7-Sox9 tm2Crm /J Jackson Laboratory Stock#: 013106, RRID:IMSR_JAX:013106 Oligonucleotides See Supplemental Table 11 for TaqMan Probe ID ThermoFisher Scientific Recombinant DNA None Software and Algorithms Transcriptome Analysis Console ThermoFisher Scientific Ingenuity Pathway Analysis QIAGEN R www.r-project.org Sequence Detection System v 2.-4. .. ThermoFisher Scientific Other ImmPACT DAB Staining Kit Vector Labs Cat#: SK-4105 Hematoxylin QS Vector Labs Cat#: H-3404 Peroxidase-conjugated Streptavidin Vector Labs Cat#: PK-6100 RNeasy Mini Kit QIAGEN Cat#: 74104 GeneChip WT Pico Reagent Kit ThermoFisher Scientific Cat#: 902623 High Capacity cDNA Reverse Transcription Kit ThermoFisher Scientific Cat#: 4368814 TaqMan Preamp Mastermix Kit ThermoFisher Scientific Cat#: 4384267 Open in a separate window Key resources Table

    SYBR Green Assay:

    Article Title: The Developmental and Genetic Architecture of the Sexually Selected Male Ornament of Swordtails.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins TRIzol Reagent Invitrogen Cat#15596026 dNTPs (Set, 100mM) Sigma-Aldrich Cat#DNTP100 DNase, RNase-free Thermo Scientific Cat#EN0521 SYBR Green I (10 000x in DMSO) Invitrogen Cat#S7563 Q5 High Fidelity DNA Polymerase New England Biolabs Cat#M04915 Critical Commercial Assays AmpliCap-Max T7 High Yield Message Maker Kit Biozym Cat#150472 RevertAid First Strand cDNA Synthesis Kit Thermo Scientific Cat#K1621 Deposited Data kcnh8 sequences this paper https://github.com/dukecomeback/swordtail/blob/main/ kcnh8_Xiphophorus.fa RNA-seq short read sequences 16 and this paper ENA accession code PRJEB8012 and PRJNA673149 Experimental Models: Organisms/Strains Xiphophorus hellerii, Rio Lancetilla strain Biocenter Würzburg WLC1337 Xiphophorus maculatus, Rio Jamapa strain Jp163A Biocenter Würzburg WLC6628 Xiphophorus montezumae, Tamasops strain Biocenter Würzburg WLC1052 Xiphophorus monticolus, El Tejon strain Biocenter Würzburg WLC3344 Xiphophorus pygmaeus, strain Rio Axtla Biocenter Würzburg WLC3015 Priapella lacandonae Biocenter Würzburg WLC5281 Oryzias latipes, Carbio strain Biocenter Würzburg WLC2674 Oligonucleotides Oligonucleotide Primers for PCR see Table S3 for primers N/A Recombinant DNA pcDNA3 Thermo Fisher V79020 pNBI16/pNB1u 66 N/A Software and Algorithms GSNAP version 2018-03-25 67 http://research-pub.gene.com/gmap/ STAR 66 https://github.com/alexdobin/STAR/releases DESeq2 68 https://bioconductor.org/packages/release/bioc/html/ DESeq2.html Ensembl Biomart 69 http://www.ensembl.org/biomart/ martview/ a61c56ebb44f17 c0e39ce71a4d79f44a DAVID 70 https://david.ncifcrf.gov/ Inparanoid 71 http://inparanoid.sbc.su.se/cgi-bin/index.cgi Pal2Nal 72 http://www.bork.embl.de/pal2nal/ MUSCLE 73 https://www.ebi.ac.uk/Tools/msa/muscle/ Gblocks 74 http://phylogeny.lirmm.fr/phylo_cgi/one_task.cgi?task_ type=gblocks Phylip 75 http://evolution.genetics.washington.edu/phylip.html, FigTree see website http://tree.bio.ed.ac.uk/software/figtree/ ETE3 76 http://etetoolkit.org/ Primer3 (version 4.4.0) see website https://bioinfo.ut.ee/primer3/ R/qtl v.1.39-5 see website https://rqtl.org/ (Continued on next page) Current Biology 31, 911–922.e1–e4, March 8, 2021 e1 ..

    cDNA Synthesis:

    Article Title: The Developmental and Genetic Architecture of the Sexually Selected Male Ornament of Swordtails.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins TRIzol Reagent Invitrogen Cat#15596026 dNTPs (Set, 100mM) Sigma-Aldrich Cat#DNTP100 DNase, RNase-free Thermo Scientific Cat#EN0521 SYBR Green I (10 000x in DMSO) Invitrogen Cat#S7563 Q5 High Fidelity DNA Polymerase New England Biolabs Cat#M04915 Critical Commercial Assays AmpliCap-Max T7 High Yield Message Maker Kit Biozym Cat#150472 RevertAid First Strand cDNA Synthesis Kit Thermo Scientific Cat#K1621 Deposited Data kcnh8 sequences this paper https://github.com/dukecomeback/swordtail/blob/main/ kcnh8_Xiphophorus.fa RNA-seq short read sequences 16 and this paper ENA accession code PRJEB8012 and PRJNA673149 Experimental Models: Organisms/Strains Xiphophorus hellerii, Rio Lancetilla strain Biocenter Würzburg WLC1337 Xiphophorus maculatus, Rio Jamapa strain Jp163A Biocenter Würzburg WLC6628 Xiphophorus montezumae, Tamasops strain Biocenter Würzburg WLC1052 Xiphophorus monticolus, El Tejon strain Biocenter Würzburg WLC3344 Xiphophorus pygmaeus, strain Rio Axtla Biocenter Würzburg WLC3015 Priapella lacandonae Biocenter Würzburg WLC5281 Oryzias latipes, Carbio strain Biocenter Würzburg WLC2674 Oligonucleotides Oligonucleotide Primers for PCR see Table S3 for primers N/A Recombinant DNA pcDNA3 Thermo Fisher V79020 pNBI16/pNB1u 66 N/A Software and Algorithms GSNAP version 2018-03-25 67 http://research-pub.gene.com/gmap/ STAR 66 https://github.com/alexdobin/STAR/releases DESeq2 68 https://bioconductor.org/packages/release/bioc/html/ DESeq2.html Ensembl Biomart 69 http://www.ensembl.org/biomart/ martview/ a61c56ebb44f17 c0e39ce71a4d79f44a DAVID 70 https://david.ncifcrf.gov/ Inparanoid 71 http://inparanoid.sbc.su.se/cgi-bin/index.cgi Pal2Nal 72 http://www.bork.embl.de/pal2nal/ MUSCLE 73 https://www.ebi.ac.uk/Tools/msa/muscle/ Gblocks 74 http://phylogeny.lirmm.fr/phylo_cgi/one_task.cgi?task_ type=gblocks Phylip 75 http://evolution.genetics.washington.edu/phylip.html, FigTree see website http://tree.bio.ed.ac.uk/software/figtree/ ETE3 76 http://etetoolkit.org/ Primer3 (version 4.4.0) see website https://bioinfo.ut.ee/primer3/ R/qtl v.1.39-5 see website https://rqtl.org/ (Continued on next page) Current Biology 31, 911–922.e1–e4, March 8, 2021 e1 ..

    RNA Sequencing:

    Article Title: The Developmental and Genetic Architecture of the Sexually Selected Male Ornament of Swordtails.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins TRIzol Reagent Invitrogen Cat#15596026 dNTPs (Set, 100mM) Sigma-Aldrich Cat#DNTP100 DNase, RNase-free Thermo Scientific Cat#EN0521 SYBR Green I (10 000x in DMSO) Invitrogen Cat#S7563 Q5 High Fidelity DNA Polymerase New England Biolabs Cat#M04915 Critical Commercial Assays AmpliCap-Max T7 High Yield Message Maker Kit Biozym Cat#150472 RevertAid First Strand cDNA Synthesis Kit Thermo Scientific Cat#K1621 Deposited Data kcnh8 sequences this paper https://github.com/dukecomeback/swordtail/blob/main/ kcnh8_Xiphophorus.fa RNA-seq short read sequences 16 and this paper ENA accession code PRJEB8012 and PRJNA673149 Experimental Models: Organisms/Strains Xiphophorus hellerii, Rio Lancetilla strain Biocenter Würzburg WLC1337 Xiphophorus maculatus, Rio Jamapa strain Jp163A Biocenter Würzburg WLC6628 Xiphophorus montezumae, Tamasops strain Biocenter Würzburg WLC1052 Xiphophorus monticolus, El Tejon strain Biocenter Würzburg WLC3344 Xiphophorus pygmaeus, strain Rio Axtla Biocenter Würzburg WLC3015 Priapella lacandonae Biocenter Würzburg WLC5281 Oryzias latipes, Carbio strain Biocenter Würzburg WLC2674 Oligonucleotides Oligonucleotide Primers for PCR see Table S3 for primers N/A Recombinant DNA pcDNA3 Thermo Fisher V79020 pNBI16/pNB1u 66 N/A Software and Algorithms GSNAP version 2018-03-25 67 http://research-pub.gene.com/gmap/ STAR 66 https://github.com/alexdobin/STAR/releases DESeq2 68 https://bioconductor.org/packages/release/bioc/html/ DESeq2.html Ensembl Biomart 69 http://www.ensembl.org/biomart/ martview/ a61c56ebb44f17 c0e39ce71a4d79f44a DAVID 70 https://david.ncifcrf.gov/ Inparanoid 71 http://inparanoid.sbc.su.se/cgi-bin/index.cgi Pal2Nal 72 http://www.bork.embl.de/pal2nal/ MUSCLE 73 https://www.ebi.ac.uk/Tools/msa/muscle/ Gblocks 74 http://phylogeny.lirmm.fr/phylo_cgi/one_task.cgi?task_ type=gblocks Phylip 75 http://evolution.genetics.washington.edu/phylip.html, FigTree see website http://tree.bio.ed.ac.uk/software/figtree/ ETE3 76 http://etetoolkit.org/ Primer3 (version 4.4.0) see website https://bioinfo.ut.ee/primer3/ R/qtl v.1.39-5 see website https://rqtl.org/ (Continued on next page) Current Biology 31, 911–922.e1–e4, March 8, 2021 e1 ..

    Polymerase Chain Reaction:

    Article Title: The Developmental and Genetic Architecture of the Sexually Selected Male Ornament of Swordtails.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins TRIzol Reagent Invitrogen Cat#15596026 dNTPs (Set, 100mM) Sigma-Aldrich Cat#DNTP100 DNase, RNase-free Thermo Scientific Cat#EN0521 SYBR Green I (10 000x in DMSO) Invitrogen Cat#S7563 Q5 High Fidelity DNA Polymerase New England Biolabs Cat#M04915 Critical Commercial Assays AmpliCap-Max T7 High Yield Message Maker Kit Biozym Cat#150472 RevertAid First Strand cDNA Synthesis Kit Thermo Scientific Cat#K1621 Deposited Data kcnh8 sequences this paper https://github.com/dukecomeback/swordtail/blob/main/ kcnh8_Xiphophorus.fa RNA-seq short read sequences 16 and this paper ENA accession code PRJEB8012 and PRJNA673149 Experimental Models: Organisms/Strains Xiphophorus hellerii, Rio Lancetilla strain Biocenter Würzburg WLC1337 Xiphophorus maculatus, Rio Jamapa strain Jp163A Biocenter Würzburg WLC6628 Xiphophorus montezumae, Tamasops strain Biocenter Würzburg WLC1052 Xiphophorus monticolus, El Tejon strain Biocenter Würzburg WLC3344 Xiphophorus pygmaeus, strain Rio Axtla Biocenter Würzburg WLC3015 Priapella lacandonae Biocenter Würzburg WLC5281 Oryzias latipes, Carbio strain Biocenter Würzburg WLC2674 Oligonucleotides Oligonucleotide Primers for PCR see Table S3 for primers N/A Recombinant DNA pcDNA3 Thermo Fisher V79020 pNBI16/pNB1u 66 N/A Software and Algorithms GSNAP version 2018-03-25 67 http://research-pub.gene.com/gmap/ STAR 66 https://github.com/alexdobin/STAR/releases DESeq2 68 https://bioconductor.org/packages/release/bioc/html/ DESeq2.html Ensembl Biomart 69 http://www.ensembl.org/biomart/ martview/ a61c56ebb44f17 c0e39ce71a4d79f44a DAVID 70 https://david.ncifcrf.gov/ Inparanoid 71 http://inparanoid.sbc.su.se/cgi-bin/index.cgi Pal2Nal 72 http://www.bork.embl.de/pal2nal/ MUSCLE 73 https://www.ebi.ac.uk/Tools/msa/muscle/ Gblocks 74 http://phylogeny.lirmm.fr/phylo_cgi/one_task.cgi?task_ type=gblocks Phylip 75 http://evolution.genetics.washington.edu/phylip.html, FigTree see website http://tree.bio.ed.ac.uk/software/figtree/ ETE3 76 http://etetoolkit.org/ Primer3 (version 4.4.0) see website https://bioinfo.ut.ee/primer3/ R/qtl v.1.39-5 see website https://rqtl.org/ (Continued on next page) Current Biology 31, 911–922.e1–e4, March 8, 2021 e1 ..

    Software:

    Article Title: The Developmental and Genetic Architecture of the Sexually Selected Male Ornament of Swordtails.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins TRIzol Reagent Invitrogen Cat#15596026 dNTPs (Set, 100mM) Sigma-Aldrich Cat#DNTP100 DNase, RNase-free Thermo Scientific Cat#EN0521 SYBR Green I (10 000x in DMSO) Invitrogen Cat#S7563 Q5 High Fidelity DNA Polymerase New England Biolabs Cat#M04915 Critical Commercial Assays AmpliCap-Max T7 High Yield Message Maker Kit Biozym Cat#150472 RevertAid First Strand cDNA Synthesis Kit Thermo Scientific Cat#K1621 Deposited Data kcnh8 sequences this paper https://github.com/dukecomeback/swordtail/blob/main/ kcnh8_Xiphophorus.fa RNA-seq short read sequences 16 and this paper ENA accession code PRJEB8012 and PRJNA673149 Experimental Models: Organisms/Strains Xiphophorus hellerii, Rio Lancetilla strain Biocenter Würzburg WLC1337 Xiphophorus maculatus, Rio Jamapa strain Jp163A Biocenter Würzburg WLC6628 Xiphophorus montezumae, Tamasops strain Biocenter Würzburg WLC1052 Xiphophorus monticolus, El Tejon strain Biocenter Würzburg WLC3344 Xiphophorus pygmaeus, strain Rio Axtla Biocenter Würzburg WLC3015 Priapella lacandonae Biocenter Würzburg WLC5281 Oryzias latipes, Carbio strain Biocenter Würzburg WLC2674 Oligonucleotides Oligonucleotide Primers for PCR see Table S3 for primers N/A Recombinant DNA pcDNA3 Thermo Fisher V79020 pNBI16/pNB1u 66 N/A Software and Algorithms GSNAP version 2018-03-25 67 http://research-pub.gene.com/gmap/ STAR 66 https://github.com/alexdobin/STAR/releases DESeq2 68 https://bioconductor.org/packages/release/bioc/html/ DESeq2.html Ensembl Biomart 69 http://www.ensembl.org/biomart/ martview/ a61c56ebb44f17 c0e39ce71a4d79f44a DAVID 70 https://david.ncifcrf.gov/ Inparanoid 71 http://inparanoid.sbc.su.se/cgi-bin/index.cgi Pal2Nal 72 http://www.bork.embl.de/pal2nal/ MUSCLE 73 https://www.ebi.ac.uk/Tools/msa/muscle/ Gblocks 74 http://phylogeny.lirmm.fr/phylo_cgi/one_task.cgi?task_ type=gblocks Phylip 75 http://evolution.genetics.washington.edu/phylip.html, FigTree see website http://tree.bio.ed.ac.uk/software/figtree/ ETE3 76 http://etetoolkit.org/ Primer3 (version 4.4.0) see website https://bioinfo.ut.ee/primer3/ R/qtl v.1.39-5 see website https://rqtl.org/ (Continued on next page) Current Biology 31, 911–922.e1–e4, March 8, 2021 e1 ..

    Article Title: The novel HLA-DQB1*03:282N allele was identified in a Chinese individual.
    Article Snippet: The HLA region contains the most polymorphic loci of the human genome and plays a significant role in immunity and disease susceptibility.. The HLA-DQB1 locus is located in the class II region of the human major histocompatibility complex, and a total of 1968 HLA-DQB1 alleles including 31 HLA-DQB1*03 null alleles have been identified in populations around the world according to the IPD-IMGT/HLA Database version 3.43.0 (January 2021).. In this report, we describe the novel allele, now named HLA-DQB1*03:282N, that has been identified in a Chinese aplastic anemia patient.

    Article Title: Cocaine-related DNA methylation in caudate neurons alters 3D chromatin structure of the IRXA gene cluster.
    Article Snippet: Genomic DNA from both cohorts (2 μg/sample), as well as from all sorted nuclear fractions (>100 ng/sample) was converted using EpiTect Fast 96 Bisulfite Conversion Kits (Qiagen, Montreal, Canada), diluted to 150 μL using MilliQ water, and stored at −20 °C. .. To optimize our ability to cover the desired CpGs within each amplicon, and to increase amplicon diversity for sequencing, we designed three pairs of bisulfite-specific primers per DNA strand using Methyl Primer Express Software v1.0 (Applied Biosystems, CA, USA). ..

    Article Title: Evaluating the contribution of the gene TARDBP in Italian patients with amyotrophic lateral sclerosis.
    Article Snippet: Peripheral blood samples were collected from patients after written informed consent and genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). .. Coding exons and flanking intronic regions of TARDBP (NM_007375.4) were sequenced on an ABI3130XL Genetic Analyzer (Applied Biosystems), using standard protocols for Sanger sequencing, and analysed using DNA Sequencing Analysis Software v.5.1. ..

    Article Title: First report of the invasive snail Pomacea canaliculata in Kenya
    Article Snippet: Sanger sequencing was undertaken using an ABI 3130 Genetic Analyser in accordance with the manufacturer’s instructions. .. Sequence trace files were examined for quality using the DNA Sequencing Analysis Software 6 (Applied Biosystems, UK), then exported as text files. ..

    Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
    Article Snippet: Reagent or resource Source Identifier Antibodies Rabbit Monoclonal Anti-SOX9 Abcam Cat# ab185230, RRID: AB_2715497 Rabbit Monoclonal Anti-CDH1 Cell Signaling Cat#: 3195, RRID: AB_2291471 Biotinylated Goat Anti-rabbit IgG Vector Labs Cat#: BA-1000, RRID: AB_2313606 Horse Anti-rabbit IgG, ImmPRESS VR Reagent Vector Labs Cat#: MP-6401, RRID: AB_2336529 Bacterial and Virus Strains None Biological Samples Goat Serum Millipore-Sigma Cat#: G9023 Bovine Serum Albumin Millipore-Sigma Cat#: 29-102-5 GM Trypsin Difco Cat#: 215240 Collagenase Millipore-Sigma Cat#: C9891 Chemicals, Peptides, and Recombinant Proteins Bluo-gal Gold Biotechnology Cat#: B-673-250 Critical Commercial Assays Agilent RNA 6000 Pico Agilent Technologies Cat#: 5067-1513 GeneChip Mouse Gene 1.0 ST Array ThermoFisher Scientific Cat#: 901171 Deposited Data RNA Microarray Data NCBI GEO Database GSE113011 ; reviewer token mhizgciwxxcxbyl Experimental Models: Cell Lines None Experimental Models: Organisms/Strains Mouse: C57BL/6J Jackson Laboratory Stock#:000664, RRID: IMSR_JAX: 000664 Mouse: B6.129S4-Gt(ROSA)26 sortm1Sor /J Jackson Laboratory Stock# 003474, RRID: IMSR_J AX: 003474 Mouse: B6. .. CgShh tm1(EGFP/cre)Cjt /J Jackson Laboratory Stock#005622; RRID: IMSR_J AX: 005622 Mouse: B6.129S7-Sox9 tm2Crm /J Jackson Laboratory Stock#: 013106, RRID:IMSR_JAX:013106 Oligonucleotides See Supplemental Table 11 for TaqMan Probe ID ThermoFisher Scientific Recombinant DNA None Software and Algorithms Transcriptome Analysis Console ThermoFisher Scientific Ingenuity Pathway Analysis QIAGEN R www.r-project.org Sequence Detection System v 2.-4. .. ThermoFisher Scientific Other ImmPACT DAB Staining Kit Vector Labs Cat#: SK-4105 Hematoxylin QS Vector Labs Cat#: H-3404 Peroxidase-conjugated Streptavidin Vector Labs Cat#: PK-6100 RNeasy Mini Kit QIAGEN Cat#: 74104 GeneChip WT Pico Reagent Kit ThermoFisher Scientific Cat#: 902623 High Capacity cDNA Reverse Transcription Kit ThermoFisher Scientific Cat#: 4368814 TaqMan Preamp Mastermix Kit ThermoFisher Scientific Cat#: 4384267 Open in a separate window Key resources Table

    other:

    Article Title: P711 Back to the future: The MHC complement genes revisited in transplant and disease
    Article Snippet: Aim: The Gamma block is in the central Major Histocompatibility Complex (MHC) region between MICB and HLA-DRB genes and contains many inflammatory and immune regulatory genes, such as C2, CFB, and C4.. The protein allotypes of these genes were important in defining the original polymorphic frozen block structure of MHC in the 1990s and were used in transplantation and disease association studies.. However, molecular assays for C2, CFB, and C4 have not been developed in parallel with HLA typing and the Gamma block genes are rarely typed today.

    Article Title: Compositions and methods for single G-level HLA typing
    Article Snippet: For example, HLA FusionTM software (One Lambda, Canoga Park, Calif.) interprets the pattern of probe hybridization or lack of hybridization by comparing probe sequences to a database of reference HLA sequences, for example, the HLA allele and sequence database found at ebi.ac.uk/ipd/imgt/hla.

    DNA Sequencing:

    Article Title: The novel HLA-DQB1*03:282N allele was identified in a Chinese individual.
    Article Snippet: The HLA region contains the most polymorphic loci of the human genome and plays a significant role in immunity and disease susceptibility.. The HLA-DQB1 locus is located in the class II region of the human major histocompatibility complex, and a total of 1968 HLA-DQB1 alleles including 31 HLA-DQB1*03 null alleles have been identified in populations around the world according to the IPD-IMGT/HLA Database version 3.43.0 (January 2021).. In this report, we describe the novel allele, now named HLA-DQB1*03:282N, that has been identified in a Chinese aplastic anemia patient.

    Article Title: Evaluating the contribution of the gene TARDBP in Italian patients with amyotrophic lateral sclerosis.
    Article Snippet: Peripheral blood samples were collected from patients after written informed consent and genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). .. Coding exons and flanking intronic regions of TARDBP (NM_007375.4) were sequenced on an ABI3130XL Genetic Analyzer (Applied Biosystems), using standard protocols for Sanger sequencing, and analysed using DNA Sequencing Analysis Software v.5.1. ..

    Article Title: First report of the invasive snail Pomacea canaliculata in Kenya
    Article Snippet: Sanger sequencing was undertaken using an ABI 3130 Genetic Analyser in accordance with the manufacturer’s instructions. .. Sequence trace files were examined for quality using the DNA Sequencing Analysis Software 6 (Applied Biosystems, UK), then exported as text files. ..

    Amplification:

    Article Title: Cocaine-related DNA methylation in caudate neurons alters 3D chromatin structure of the IRXA gene cluster.
    Article Snippet: Genomic DNA from both cohorts (2 μg/sample), as well as from all sorted nuclear fractions (>100 ng/sample) was converted using EpiTect Fast 96 Bisulfite Conversion Kits (Qiagen, Montreal, Canada), diluted to 150 μL using MilliQ water, and stored at −20 °C. .. To optimize our ability to cover the desired CpGs within each amplicon, and to increase amplicon diversity for sequencing, we designed three pairs of bisulfite-specific primers per DNA strand using Methyl Primer Express Software v1.0 (Applied Biosystems, CA, USA). ..

    Sequencing:

    Article Title: Cocaine-related DNA methylation in caudate neurons alters 3D chromatin structure of the IRXA gene cluster.
    Article Snippet: Genomic DNA from both cohorts (2 μg/sample), as well as from all sorted nuclear fractions (>100 ng/sample) was converted using EpiTect Fast 96 Bisulfite Conversion Kits (Qiagen, Montreal, Canada), diluted to 150 μL using MilliQ water, and stored at −20 °C. .. To optimize our ability to cover the desired CpGs within each amplicon, and to increase amplicon diversity for sequencing, we designed three pairs of bisulfite-specific primers per DNA strand using Methyl Primer Express Software v1.0 (Applied Biosystems, CA, USA). ..

    Article Title: Evaluating the contribution of the gene TARDBP in Italian patients with amyotrophic lateral sclerosis.
    Article Snippet: Peripheral blood samples were collected from patients after written informed consent and genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). .. Coding exons and flanking intronic regions of TARDBP (NM_007375.4) were sequenced on an ABI3130XL Genetic Analyzer (Applied Biosystems), using standard protocols for Sanger sequencing, and analysed using DNA Sequencing Analysis Software v.5.1. ..

    Article Title: First report of the invasive snail Pomacea canaliculata in Kenya
    Article Snippet: Sanger sequencing was undertaken using an ABI 3130 Genetic Analyser in accordance with the manufacturer’s instructions. .. Sequence trace files were examined for quality using the DNA Sequencing Analysis Software 6 (Applied Biosystems, UK), then exported as text files. ..

    Article Title: Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration
    Article Snippet: Reagent or resource Source Identifier Antibodies Rabbit Monoclonal Anti-SOX9 Abcam Cat# ab185230, RRID: AB_2715497 Rabbit Monoclonal Anti-CDH1 Cell Signaling Cat#: 3195, RRID: AB_2291471 Biotinylated Goat Anti-rabbit IgG Vector Labs Cat#: BA-1000, RRID: AB_2313606 Horse Anti-rabbit IgG, ImmPRESS VR Reagent Vector Labs Cat#: MP-6401, RRID: AB_2336529 Bacterial and Virus Strains None Biological Samples Goat Serum Millipore-Sigma Cat#: G9023 Bovine Serum Albumin Millipore-Sigma Cat#: 29-102-5 GM Trypsin Difco Cat#: 215240 Collagenase Millipore-Sigma Cat#: C9891 Chemicals, Peptides, and Recombinant Proteins Bluo-gal Gold Biotechnology Cat#: B-673-250 Critical Commercial Assays Agilent RNA 6000 Pico Agilent Technologies Cat#: 5067-1513 GeneChip Mouse Gene 1.0 ST Array ThermoFisher Scientific Cat#: 901171 Deposited Data RNA Microarray Data NCBI GEO Database GSE113011 ; reviewer token mhizgciwxxcxbyl Experimental Models: Cell Lines None Experimental Models: Organisms/Strains Mouse: C57BL/6J Jackson Laboratory Stock#:000664, RRID: IMSR_JAX: 000664 Mouse: B6.129S4-Gt(ROSA)26 sortm1Sor /J Jackson Laboratory Stock# 003474, RRID: IMSR_J AX: 003474 Mouse: B6. .. CgShh tm1(EGFP/cre)Cjt /J Jackson Laboratory Stock#005622; RRID: IMSR_J AX: 005622 Mouse: B6.129S7-Sox9 tm2Crm /J Jackson Laboratory Stock#: 013106, RRID:IMSR_JAX:013106 Oligonucleotides See Supplemental Table 11 for TaqMan Probe ID ThermoFisher Scientific Recombinant DNA None Software and Algorithms Transcriptome Analysis Console ThermoFisher Scientific Ingenuity Pathway Analysis QIAGEN R www.r-project.org Sequence Detection System v 2.-4. .. ThermoFisher Scientific Other ImmPACT DAB Staining Kit Vector Labs Cat#: SK-4105 Hematoxylin QS Vector Labs Cat#: H-3404 Peroxidase-conjugated Streptavidin Vector Labs Cat#: PK-6100 RNeasy Mini Kit QIAGEN Cat#: 74104 GeneChip WT Pico Reagent Kit ThermoFisher Scientific Cat#: 902623 High Capacity cDNA Reverse Transcription Kit ThermoFisher Scientific Cat#: 4368814 TaqMan Preamp Mastermix Kit ThermoFisher Scientific Cat#: 4384267 Open in a separate window Key resources Table



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